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RBGKEW1309 Long-Read Ash Genome Sequencing 2024

Royal Botanic Gardens, Kew Published 22 Aug 2024 Contracts Finder

key details

Value£167,000
Statuscomplete
Category (CPV) 73110000
RegionLondon
Deadline13 Jun 2024
Procedureopen
OCIDocds-h6vhtk-045914

Awards (2)

This procurement was awarded to 1 suppliers. Values shown are per-award; the notice total is £167,000.

SupplierValueDateStatus
Novogene (UK) Company LTD £150,000 25 Jul 2024 active
Novogene (UK) Company LTD · · active

description

Royal Botanic Gardens, Kew wishes to appoint a supplier to individually sequence the whole genomes of up to 300 European ash trees (Fraxinus excelsior) at a minimum of 15x whole genome coverage via a long-read technology. Fraxinus excelsior is a diploid and has a 1C genome size of 880 Mbp.

In addition to the 300 individual ash trees, we wish to sequence a minimum of six technical replicates. Therefore, the total number of samples we wish to sequence is 306.

RBG Kew intends to supply total genomic DNA for each sample with the following metrics: minimum of 10ug DNA; minimum concentration of 30ng/ml (as measured with a Quantus flurometer dsDNA assay); at least 50% of the sample in fragments with a minimum length of 40kb; minimum OD260/280 of 1.8; minimum OD260/230 of 1.8; minimum DIN of 7.

documents

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notice history

4 notices published against this procurement.

PublishedTypeRegimeNotice
13 May 2024 Contract notice (F02) Earlier regulations 015129-2024
13 May 2024 Tender (tender) · bf18ab55-3acc-468c-9191-46e41757cedc-750320
22 Aug 2024 Contract award notice (F03) Earlier regulations 026781-2024
22 Aug 2024 Award (award) · c1725ab5-9241-4833-b1ea-aa636dc877f6-779501

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