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MIROCALS Study Flow cytometry cell subset numeration in human whole blood and monitoring of pNF-H in CSF and plasma samples by ELISA

University of Sussex Published 21 Jul 2016 Contracts Finder

key details

Statusactive
Category (CPV) 73111000
RegionSouth East
Deadline19 Aug 2016
Contract start19 Sept 2016
Contract end18 Sept 2018
Procedureopen
SME suitableNo
OCIDocds-b5fd17-ed227063-adc9-11e6-9901-0019b9f3037b

description

The Service Provider is requested to monitor the absolute number and frequency of circulating white blood cell subsets including FOXP3+ regulatory T cells (Tregs), CD3+, CD4+, CD8+, B, and NK cells in EDTA whole blood samples and the circulating pNF-H biomarker in CSF and plasma.

For whole blood samples, cell subsets should be analysed by flow cytometry. There will be 216 patients and 5 time points per patient (1080 samples in total).

For plasma and CSF samples, the pNF-H should be monitored using a commercial ELISA kit (Biovendor). There will be 3 plasma samples + 3 CSF samples per patient from 216 patients (2 x 3 x 216 = 1296 samples in total.

All procedures will need to be validated, including the preanalytical stability of whole blood samples prior to flow cytometry analysis.

Additional information: Is a Recurrent Procurement Type? : No https://in-tendhost.co.uk/universityofsussex/aspx/

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notice history

1 notice published against this procurement.

PublishedTypeRegimeNotice
18 Nov 2016 Tender (tender) · b5853e3b-3e8f-4274-85cb-4acecc067b03-103313

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