RQ0000010125 Epifluorescence Microscope
key details
| Value | £100,000 |
|---|---|
| Status | complete |
| Category (CPV) | 38515200 |
| Deadline | 13 Apr 2023 |
| Contract start | 30 Apr 2023 |
| Contract end | 31 May 2023 |
| Procedure | open |
| SME suitable | Yes |
| OCID | ocds-b5fd17-e6585b21-3b0d-4d31-8c28-8f3dc8fa7ec1 |
Award
| Supplier | Value | Date | Status |
|---|---|---|---|
| CARL ZEISS LTD | £172,121 | 4 Oct 2023 | active |
description
Intended uses (priority order):
- Real-time imaging of cellular physiology using ion-sensitive fluorophores or similar probes (e.g. fura 2 and fluo dyes with ability for acquisition of fura 2 ratiometric images ≥1Hz)
- General epifluorescence imaging of living cell cultures using a range of fluorescent dyes to image cellular structure and function (cell viability, mitochondrial function, ROS etc). Excitation/emission capabilities should cover standard range of fluorophores (e.g. DAPI, fluorescein, rhodamine etc).
- General transmitted light and epifluorescence imaging of fixed / stained cell cultures and slide-mounted tissue sections
Essential requirements:
| • | Research-grade inverted microscope chassis, stage, transmitted light illumination, eyepieces etc |
| • | Microscope should be capable of phase and differential interference contrast transmitted light methods. |
| • | Objectives 10, 20, 40 and ~60 or 100x optimised for epifluorescence observation and DIC, 20 and 40x objectives optimised for UV excitation for Fura 2 (@340 & 380 nm, if different from standard epifluorescence objectives), additional 20 and 40x for phase contrast. |
| • | Epifluorescence light source to provide illumination for cellular physiology studies (excitation suitable for a range of dyes including fura 2) should include software controlled shutter, or equivalent, to control illumination and synchronise to image acquisition. |
| • | Epifluorescence light source for general epifluorescence imaging (2 and 3 above) if different |
| • | Ability to mount both epifluorescence illumination systems and switch between physiology and high-resolution epifluorescence imaging modes |
| • | Monochrome camera suitable for real-time imaging cellular physiology |
| • | Monochrome camera for high resolution general epifluorescence (if different from above) |
| • | Ability to mount both cameras and switch between physiology and high-resolution epifluorescence imaging modes |
| • | Image acquisition and analysis software suitable for running cellular physiology studies |
| • | Image acquisition and analysis software suitable for general epifluorescence studies. Ability to combine fluorescence images to produce composite images, ability to undertake image analysis/cytometry measurements of fluorescently labelled specimens. |
Desirable requirements:
| • | Motorised control of microscope operation |
| • | Additional image analysis options for analysis of standard histopathology tissue sections |
Other:
- If all capability is not possible on one microscope chassis then would consider two systems.
This requirement will be on the defence sourcing portal (DSP)
Additional information: How to apply - This notice can be found on the defence sourcing portal at this link: https://www.contracts.mod.uk/go/172903630186FEB197D9
documents
- https://www.contractsfinder.service.gov.uk/Notice/Attachment/334ef375-e903-4f99-a521-b5d50385eecc , tenderNotice
- https://www.contractsfinder.service.gov.uk/Notice/Attachment/56c38300-7977-45e5-a30f-e1e9ebd84d6a , tenderNotice
- https://www.contractsfinder.service.gov.uk/Notice/b5f32404-39e2-4b9f-b5ea-77a5d3d6a29c , tenderNotice
- https://www.contracts.mod.uk/go/172903630186FEB197D9 , contractNotice
Documents are linked, not mirrored. They are served by the publishing authority and may require registration.
notice history
3 notices published against this procurement.
| Published | Type | Regime | Notice |
|---|---|---|---|
| 27 Mar 2023 | Tender amendment (tenderAmendment) | · | b5f32404-39e2-4b9f-b5ea-77a5d3d6a29c-628374 |
| 5 Jun 2023 | Tender amendment (tenderAmendment) | · | b5f32404-39e2-4b9f-b5ea-77a5d3d6a29c-650720 |
| 23 Oct 2023 | Award (award) | · | cefdfb5f-cb3e-4062-919e-ca51726538c5-689138 |
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source
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